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This drug is indicated for the following cases such as:​

involved in the formation of new cells​

involved in the metabolism of ribonucleic acids (RNA) and deoxyribonucleic acids (DNA),​

essential for protein synthesis, essential during pregnancy to reduce the risk of neural tube defects​

essential for the normal growth and the development of the foetus​

involved in some functions associated with Vitamin B12​

necessary for building resistance to diseases in the thymus gland of new born babies and infants may reduce the risk of cervical dysplasia​

necessary for red blood cell production​

It should be noted that the administration of Folic acid alone is an improper therapy for pernicious anaemia and other megaloblastic anaemias in which vitamin B12 is deficient.​

A potential danger exist in administering Folic acid to patients with undiagnosed anemia, since Folic Acid may obscure the diagnosis of pernicious anemia by alleviating the hematologic manifestations of the disease while allowing the neurologic complications to progress.​